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Home > PUBLICATIONS > JOURNAL > JH22/2 > Abstract


Pengujian Ketahanan Klon-klon Hasil Silangan Tanaman Kentang Transgenik dengan Nontransgenik terhadap Penyakit Hawar Daun Phytophthora infestans di Lapangan Uji Terbatas
Ambarwati, AD1), Herman, M1), Listanto, E1), Suryaningsih, E2), dan Sofiari, E2)
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Balai Penelitian Tanaman Sayuran,
[Tahun Terbit : 2012]



ABSTRACT
Transgenic potato Katahdin event SP904 and  SP951 containing RB gene, which were isolated from a wild diploid potato species, Solanum bulbocastanum. RB gene showed durable resistance with broad spectrum to all known races of  P. infestans in the USA. In development of  potato resistant to late blight P. infestans in Indonesia, Katahdin transgenic were used as a resistant donor and crossed with susceptible varieties i.e. Atlantic and Granola. Clones derived from the crossing were molecularly analyzed and had RB gene contain. Experiment was conducted to assess the resistance of the clones derived from crossing of Katahdin transgenic and nontransgenic to P. infestans in confined field trial (CFT), located at the Indonesian Vegetable Research Institute (IVEGRI), Lembang from October 2009 to March 2010. Several clones tested were 12 clones of Atlantic x Katahdin transgenic SP904 (A); 15 clones of Atlantic x Katahdin transgenic SP951 (B); 17 clones of Granola x Katahdin transgenic SP904 (C); and 20 clones of Granola x Katahdin transgenic SP951 (D). Atlantic and Granola were used as susceptible control whereas Katahdin transgenic as resistant control. Observation was started as late blight symptoms and detected at 26, 32, 39, 46, and 53 days after planting. Plant resistance decreases with increasing period of observation, followed by increasing disease intensity and AUDPC. All clones tested showed variation in phenotypic resistance to late blight P. infestans. Clones derived from crossing of Atlantic x Katahdin transgenic SP951 had AUDPC score 697 and almost similar to Katahdin transgenic SP904 (698.5). Clones derived from crossing of Granola x Katahdin transgenic SP951 had AUDPC score 687.5 and smaller than Katahdin transgenic SP904. The results also indicated that these clones had higher resistance than Katahdin transgenic SP904. Observation at 46 days after planting or 20 days after infection resulted three resistant clones i.e. B49 (score 7.5), C111 (score 7.1); and D26 (score 7.3).  This resistance was higher than Katahdin transgenic SP904 (score 5.1) and Katahdin transgenic SP951 (score 6.4).

Keywords: Transgenic potato; Crossing; RB gene; Late blight


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